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About Multiplex Automated Genomic Engineering (MAGE):造成巨大的突变Virtual Lab Simulation
Imagine you were tasked to edit over 100 genes to find which gene or combinations of genes will work best to produce the pigment beta-carotene. You could try editing them one at a time, but that would take way too much time in the lab! That’s where Multiplex Automated Genome Engineering, or MAGE, comes in. Instead of painstakingly editing one gene at a time, the MAGE technique helps scientists to perform many genetic mutations at many target sites at a time.
Introduction to MAGE and designing oligos
在此模拟中，将向您介绍法师作为重组工程工具的原理，用于大规模编程和加速细胞的演变。在了解该技术的基础知识之后，您将被任命执行法师以增强β-胡萝卜素代谢途径大肠杆菌。To start your MAGE journey, you will first learn how to design the optimum oligos to be used for editinge.coligenomes.
Performing MAGE
Once you’ve understood the principles behind MAGE, you will have the freedom to experiment with changing up different parameters of the technique as you progress. For example, you can try tweaking cell density, growth media electroporation process, and MAGE cycle. Your decision will determine the outcome of your experiment!
Plating and screening
为了帮助您可视化该技术的分子级别上发生的情况，该模拟向您显示了沉浸式3D动画中法师周期的分步进展。您在模拟开始时设计实验时做出的选择将在结果的筛选步骤中描绘e.coli克隆。
您是否可以增强β-胡萝卜素的生产E. colito help improve the eyesight of young children?
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Gain insight into how scientists can improve children’s eyesight by genetically modifying E.coli to produce more beta-carotene.
Techniques in Lab
- MAGE (Multiplex Automated Genome Engineering)
- Electroporation
Learning Objectives
At the end of this simulation, you will be able to…
- Explain the concept and molecular mechanism of MAGE technique
- Describe the oligo design requirements for MAGE
- Understand which proteins, enzymes, and plasmids are involved in MAGE
- Perform MAGE cycles
Simulation Features
Length– 38 minutes
Languages– English
Examples of Related Standards
University
生命科学和遗传学与第二年第三年有关
NGSS
No direct alignment
IB
No direct alignment
AP
No direct alignment
Screenshots of Multiplex Automated Genomic Engineering (MAGE):造成巨大的突变Virtual Lab Simulation
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